Fig. 1: Artificial control of HCV IRES-mediated translation through structure modulation. | Nature Communications

Fig. 1: Artificial control of HCV IRES-mediated translation through structure modulation.

From: Rational design of microRNA-responsive switch for programmable translational control in mammalian cells

Fig. 1

a Schematic illustration of DS inhibition effect of the IRES translation activity. b Reduction of IRES-mediated tagBFP translation with different length of DS. c Schematic illustration of RS recovery effect of the IRES translation activity. d Recovery of IRES-mediated tagBFP translation with different length of RS. e Representative microscopy images of nIRES, dIRES, and rIRES. Each experiment was repeated three times independently with similar results. f Representative flow cytometry scatter plots of nIRES, dIRES, and rIRES. Each experiment was repeated three times independently with similar results. HEK293 cell line was used for the transfection experiments. Data are presented as mean values with error bars representing the standard deviation of three independent biological replicates (n = 3 in each group). DS, distorting sequence. RS, rescuing sequence. nIRES, native IRES. dIRES, nIRES with 20-nt DS. rIRES, dIRES with 13-nt RS. Schematic illustration figures were created with BioRender.com with publication licenses. Source data are provided as a Source Data file.

Back to article page