Fig. 2: Pathological αSyn accumulates in PD mice ilea.

a Representative immunofluorescence images of TH (yellow), αSyn (magenta), and DAPI (blue) without (−PK) or with (+PK) Proteinase K (PK) treatment in the SN (top) or the ileum (bottom) of hαSyn mice 5 weeks following the injection. b Quantification of the MFI of αSyn in the +PK SN of EV and hαSyn mice at 1, 5, and 10 weeks following AAV injection. c Quantification of the αSyn⁺ profiles in villi following PK treatment in the ileum of EV or hαSyn animals at 1, 5, and 10 weeks following viral vector injection. d Representative image of HA (green), pαSyn (magenta), and DAPI (blue) in the ileum of an hαSyn animal five weeks following viral vector injection. e Quantification of the number of pαSyn⁺ villi in the ileum of the EV and hαSyn animals at 1, 5, and 10 weeks following viral vector injection. N numbers are indicated below each graph. All graphs depict EV (blue) and hαSyn (red). Scale bars represent 50 μm in larger images and 10 μm in the insert. Statistical analysis by two-way ANOVA with Bonferroni’s post hoc test. Data are presented as mean values +/− SEM. Source data are provided as a Source Data file.