Fig. 4: The ileum and brain share a unique microglia-like CD11c⁺ population.

a UMAP of the different CD11c⁺ single-cell transcriptome subpopulations identified in the spleen, brain, and ileum from EV and hαSyn animals. b Heatmap demonstrating the top 20 markers identified in each CD11c⁺ luster. Clusters are color-coded along the top of the graph using the same colors in (a). c UMAP as in (a) demonstrating the cells partitioned by cellular organ of origin: distinct clustering of the CD11c⁺ cells from brain (brown), ileum (magenta), or spleen (gray) of EV and hαSyn animals. d Bar graph displaying the relative percent of each CD11c⁺ cluster in the spleen, ileum, and brain of EV or hαSyn animals. e Bar graph of the top ten upregulated GO terms enriched in the TRM 1 cluster. f Dotplot demonstrating the average expression of select pro-inflammatory genes present in the top 5 GO Terms described in (e). Sequencing data is from the pooled organs of 5 EV and 5 hαSyn animals harvested 5 weeks following viral vector injection.