Fig. 2: Identification of protein domains involved in ERG-SND1/MTDH interactions.
From: SND1 binds to ERG and promotes tumor growth in genetic mouse models of prostate cancer
a Co-IP experiment between endogenous MTDH and ERG proteins in VCaP cells transfected with non-targeting control (-) or SND1 targeting (#1 and #2) siRNA oligos. b Co-IP experiment between endogenous SND1 and ERG proteins in VCaP cells transfected with non-targeting control (-) or MTDH targeting (#3 and #4) siRNA oligos. c Co-IP experiment between epitope-tagged ERG, SND1, and MTDH proteins in HEK293 cells. d, e Co-IP experiments between indicated fragments of epitope-tagged ERG and SND1 in HEK293 cells. f Schematic representation of Halo-tagged ERG proteins used in (d, e) and their interaction with full-length SND1. g, h Co-IP experiments between indicated fragments of SND1-V5, HA-ERG (g) or HALO-∆C-ERG (h) in HEK293 cells. i Schematic representation of V5-tagged SND1 proteins used in (g, h) and their interaction with ERG. kD, kilodalton. Experiments were repeated 2 (g, h), 3 (c) or 4 (d, e) times with similar results. Source data are provided as a Source data file.
