Fig. 5: Testing of various epigenetic drugs.
From: Delineating the interplay between oncogenic pathways and immunity in anaplastic Wilms tumors
a Assessment of IC50 for Wilms tumor cell line (17.94) when exposed to different epigenetic drugs at a range of concentration for 72 h. The bar plot demonstrates ranked IC50 (nM) for different inhibitors in the 17.94 cell line (n = 4 independent experiments). b Dose-response effects of drugs combined treatment on the viability of the 17.94 cell line (n = 4 independent experiments). Clonogenic survival assay results depicted as c dot bar plot and d representative images showing the number of colonies in 17.94 cell line treated or untreated with various inhibitors (n = 9 biological samples, each with 3 technical replicates). Data was presented as mean ± standard deviation, and a two-sided Mann-Whitney test was applied to measure the P values between the control cells (DMSO) and the cells treated with various inhibitors. RT-qPCR was preformed to measure the mRNA levels for e, f chemokines (CXCL9 and CXCL10); g MHC class I surface receptor expression and h PD-L1 expression in 17.94 WT cells either stimulated or left unstimulated with IFN-γ in the absence/presence of various inhibitors (n = 2 independent experiments, with triplicates). Data was presented as mean ± standard deviation. Source data are provided as a Source Data file.
