Fig. 2: Hematopoietic extrinsic effects of OVOL2 on lymphocyte development.
From: OVOL2 sustains postnatal thymic epithelial cell identity
A–D Representative flow cytometry plots (A, C) and quantitative analysis (B, D) showing repopulation of lymphocytes in the blood (A, B) or thymus (C, D) 12 wk after bone marrow transplantation (BMT) from WT (CD45.2) or Ovol2C120Y/− (CD45.2) mice to lethally irradiated CD45.1; Rag2−/− recipients. E–H Representative flow cytometry plots (E, G) and quantitative analysis (F, H) showing repopulation of lymphocytes in the blood (E, F) or thymus (G, H) 12 wk after BMT from congenic WT (C57BL/6 J; CD45.1) mice to lethally irradiated WT (CD45.2) or Ovol2C120Y/− (CD45.2) recipients. Numbers adjacent to outlined regions indicate representative percentage in each population (A, C, E) or means ± SD (G) (n = 5 recipients per group in (A, B), 4 recipients per group in (C, D), 4 C120Y/− recipients or 3 WT recipients in (E, F), 3 recipients per group in (G, H)). I–M Homeostatic expansion of T cells. Representative flow cytometric histograms and frequency of proliferated dye-positive T cells in the spleen of irradiated recipients, 7 d after transfer (n = 5 recipients per group in (I, J), 4 recipients per group in (L, M)). I, J CellTrace Far Red-stained pan T cells isolated from the spleen of Ovol2C120Y/− or WT littermates were adoptively transferred into sublethally irradiated (IR, 8.5 Gy) or unirradiated (Un-IR) WT hosts (CD45.1). J Mean fluorescence intensity (MFI) of CellTrace Far Red positive-cells. K CellTrace Violet-stained pan T cells isolated from the spleen of WT (CD45.1) mice were transferred into sublethally irradiated (8.5 Gy) Ovol2C120Y/− or WT littermates. L, M WT pan T cells isolated from WT→Ovol2+/+;Rag2−/− bone marrow chimeras or Ovol2C120Y/− pan T cells isolated from Ovol2C120Y/−→Ovol2+/+;Rag2−/− bone marrow chimeras were stained by CellTrace Far Red, and were transferred into sublethally irradiated (8.5 Gy) WT recipients (CD45.1). M MFI of CellTrace Far Red positive-cells. Data are representative of two independent experiments. Data points represent individual mice; means ± SD are indicated. P-values were determined by two-tailed Student’s t test. +/+ indicates WT and C120Y/− indicates the Ovol2C120Y/− genotype.
