Fig. 5: CCER1 self-assembles to form condensates.
From: Phase-separated CCER1 coordinates the histone-to-protamine transition and male fertility
a CCER1 forms condensed granules in the DAPI-light chromatin region of elongating spermatids (left, step 9; right, step 10) in vivo. Scale bar: 1 μm. b Large condensed granules were found in HEK293T cells transfected with a Ccer1-expressing plasmid in vitro. Scale bar: 10 μm. c Coimmunoprecipitation of Flag-CCER1 and mCherry-CCER1 from HEK293T cells expressing different CCER1-tagged proteins (Flag, mCherry, or GFP) and immunoblotted with anti-mCherry anti-Flag and anti-GFP antibodies, respectively. d Immunofluorescence verification of the colocalization of mCherry-CCER1 and GFP-CCER1 in HEK293T cells transfected with human and mouse s coding sequences. Cells with only GFP transfection were controls. Scale bar: 10 μm. e Plasmid constructs expressing full-length CCER1 (1–1212 bp), CCER1-N (1–873 bp), CCER1-C (C terminal, 1060–1212 bp), CCER1-△CC (internal deletion; 874–1059 bp), CCER1-CC (coiled-coil ___domain, 875–1058 bp) or GFP alone. f Granular condensate or punctate distribution in transfected HEK HEK293T cells. Scale bar: 10 μm.
