Fig. 6: The growth supporting oxygen-tolerant reductive glycine pathway in K. phaffii. | Nature Communications

Fig. 6: The growth supporting oxygen-tolerant reductive glycine pathway in K. phaffii.

From: The oxygen-tolerant reductive glycine pathway assimilates methanol, formate and CO2 in the yeast Komagataella phaffii

Fig. 6

The oxygen-tolerant reductive glycine pathway is the sole, natively-active alternative methanol assimilation pathway in the yeast K. phaffii; and is also the sole, native formate and native CO2 assimilation pathway. It is initiated via methanol or formate assimilation and continues via methylene-THF, glycine and serine towards the formation of pyruvate and even further towards the formation of oxaloacetate. With the deletion of SHM1 DAS1&2 double knockout strains can grow via the displayed and compartmentalized pathway without any overexpressions. Abbreviations: THF tetrahydrofolate; TCA tricarboxylic acid cycle; XuMP xylulose 5-phosphate pathway; PPP pentose phosphate pathway.

Back to article page