Fig. 4: Selectivity mechanism of latanoprost-FA toward FP receptor.

a Two-dimensional schematic depiction of the latanoprost-FA binding pocket on the FP receptor. H-bonds, salt bridges and π-π interactions are displayed as blue, orange and green dash lines respectively. Solid lines represent hydrophobic interfaces. b Comparison of the ω-chain binding sub-pocket between the FP receptor (blue) and EP3 receptor (gray). The only difference is at residue 6.51, which is a phenylalanine in the FP receptor and a leucine in the EP3 receptor. Latanoprost-FA (c) and carboprost (d)-mediated signaling on the wild-type FP, EP3 receptor, and FP (F265^6.51L) and EP3 (L298^6.51F) mutants. Values represent the means ± SD of 3 independent samples. e The ΔpEC50 of carboprost and latanoprost-FA bound to FP (F265^6.51L) and EP3 (L298^6.51F) from the wild-type receptors. Values represent the means ± SD of 3 independent samples. The significance of the value was determined using two tailed Student’s t test. **P < 0.001, ***P < 0.0001. Exact p values and Source data are provided as a Source Data file.