Fig. 1: Flow charts of field experiments for peanut disease investigation, identification of potential pathogens and pot experiment.
From: Crop rotation and native microbiome inoculation restore soil capacity to suppress a root disease
a From 2012, experimental plots were managed under two cropping regimes (treatments): peanut monocropping and rotation. For monocropping, peanuts were continuously planted from 2012 to 2018. For rotation, peanut was grown first (2012), and then maize (Zea mays L.), potato (Solanum tuberosum), and soybean (Glycine max) were ordinally planted in every other peanut planting year. The disease index of peanut was investigated at the flowering stage in 2012, 2014, and 2016. At 2018, peanut disease was investigated at seedling, flowering, and pod-bearing stage, and the rhizosphere soil was sampled for community analysis. b Roots from mature monocropped plants with healthy and diseased were used for pathogenic community analysis and potential pathogen identification. c During the end of 2018 planting season, soil samples were collected from six plots and used for pot experiments. The rhizosphere soil was collected at the seedling stage for culturable microbiome–pathogen interaction, community analysis, and functional analysis.
