Fig. 2: PZA inhibits PARP1 enzymatic activity in macrophages. | Nature Communications

Fig. 2: PZA inhibits PARP1 enzymatic activity in macrophages.

From: Inhibition of host PARP1 contributes to the anti-inflammatory and antitubercular activity of pyrazinamide

Fig. 2

a Schematic overview. PMA-differentiated THP-1 cells were incubated with 100 or 250 µM PZA, pyrazinoic acid (POA), NAM or nicotinic acid (NA), or with 10 or 25 nM of the PARP1/2 inhibitor talazoparib (Tp), for 60 min before the addition of PARP1/2 activator MNNG (5 µM). b Representative Western blot showing PAR levels (top) or the loading control β-Actin (bottom). c Densitometric analysis showing the fold change in ß-Actin-normalized PAR levels relative to cells treated with MNNG alone. UNT is untreated (no MNNG). Error bars represent the SEM from seven independent experiments (individual values indicated by scatter plot). ***p = 0.0002 (PZA high dose) or 0.0001 (NAM low dose); ****p < 0.0001; ns not significant; by repeated measures (RM) one-way ANOVA with Dunnett’s multiple comparisons test. PAR levels in cells treated with 250 µM PZA (22.9%) were significantly lower than with 100 µM PZA (43.1%) but not statistically different from cells treated with 100 µM NAM (14.6%). Source data are provided as a Source Data file.

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