Fig. 5: Expression patterns of KNOXI genes and CaLFY in the vegetative shoot meristem of chickpea.

RNA in situ hybridization with CaSTMa (a, b), CaBP1(c, d) or CaLFY (e, f) specific probes on longitudinal (a, c, e) and transverse (b, d, f) sections of 4-week-old WT vegetative-shoots. Similar results were obtained from three independent experiments. Scale bars, 50 μm. g RT–qPCR analysis of CaLFY mRNA expression levels at different leaf developmental stages. Data shows mean ± SD of 4 biological replicates. Different letters above bars indicate significant differences using the two-sided unpaired Student’s t test (p < 0.05). h RT–qPCR analysis of CaLFY expression in different portions of the P5 leaf primordia of WT. PP, Proximal Portion; DP, Distal Portion. Data shows mean ± SD of 4 biological replicates. The value above column represents the p-value estimated by the two-sided unpaired Student’s t test. i A schematic illustration of CaSTMa and CaBP1 expression pattern in the shoot apex. j A schematic illustration of CaLFY expression pattern in the shoot apex. Source data for Fig. 5g, h are provided as a Source Data file.