Fig. 6: 13-HODE promoted lipid accumulation in hepatocytes by inhibiting CAT.

a–d Primary mouse hepatocytes were transfected with Cat-expressing plasmid with or without 13-HODE treatment. a Western blot analysis of protein level of CAT. b Oil Red O staining; scale bar = 50 μm. Western blot analysis of protein levels of cleaved SREBP1 (c) and FASN (d). e–h Primary mouse hepatocytes were transfected with si-Cat or si-NC. e Western blot analysis of protein level of CAT. f Oil Red O staining; scale bar = 50 μm. Western blot analysis of protein levels of cleaved SREBP1 (g) and FASN (h). i–k Primary mouse hepatocytes were treated 13-HODE (1 μM) with or without NAC (10 mM). i Oil Red O staining; scale bar = 50 μm. Western blot analysis of protein levels of cleaved SREBP1 (j) and FASN (k). a–d, f–k n = 5 independent experiments; (e) n = 3 independent experiments. Data are presented as the mean ± SEM. Two-tailed Student’s t test was performed for (a, e, g, h); One-way ANOVA with Fisher’s LSD was performed for c, d, j, k. CAT OE catalase overexpression.