Fig. 3: TslA has phospholipase A1 activity, which is inhibited by the immunity protein, TilA.
From: A type VII-secreted lipase toxin with reverse ___domain arrangement
a, b Purified TslA and TslA with point mutants in the active site were incubated with (a) the PLA1 substrate PED-A1 or (b) the PLA2 substrate PED6. Fluorescence released upon substrate hydrolysis was measured at 515 nm over the course of 1 h. Data are presented as the mean ± SD. RFU—relative fluorescence units. c Calorimetric titration of TslA with TilA. (Upper) Raw data for the heat effect during titration. DP—differential power. (Lower) Binding isotherm. The best fit to the data gave n = 0.88 ± 0.01 binding sites, ΔH = −20.5 ± 0.8 kcal mol−1. d Hydrolysis of PED-A1 mediated by TslA alone, TilA alone or TslA and TilA at the indicated molar ratios. Negative control values were subtracted from each condition. The data presented in (a), (b) and (d) is n = 3 technical replicates. These experiments were repeated three times, with similar results observed each time. Error bars are ± SD. Source data are provided as a Source Data file.
