Fig. 6: Analysis of S. aureus membrane lipids following intoxication by TslA.
From: A type VII-secreted lipase toxin with reverse ___domain arrangement
USA300 and USA300 Δtil1 harbouring pRAB11 encoding TslA-TlaA1-TlaA2, were cultured for 2 h after which 500 ng ml−1 ATc was added to induce plasmid-encoded gene expression. Samples were subsequently withdrawn at 2 and 6 h post-induction and membranes prepared as described in methods. Mass spectrometric analysis, in negative ion mode (100-1000 m/z) was carried out on membranes for a. USA300 pRAB11 and b. USA300 TslA-TlaA1-TlaA2 after 2 h post-induction, c. USA300 Δtil1 TslA-TlaA1-TlaA2 after 2 h post-induction and d. USA300 Δtil1 TslA-TlaA1-TlaA2 after 6 h post induction.
