Fig. 1: Functional expression of the reductive TCA cycle in Y. lipolytica. | Nature Communications

Fig. 1: Functional expression of the reductive TCA cycle in Y. lipolytica.

From: Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica

Fig. 1

a Construction of the reductive TCA cycle in SDH-deficient Y. lipolytica strain. Red arrows represent the oxidative TCA cycle, and blue arrows represent the reductive TCA cycle. Red X represents the deficiency of succinate dehydrogenase. OAA oxaloacetate, MA malate, FA fumarate, SA succinate, NADH nicotinamide adenine dinucleotide. b Evaluating different Frds on SA synthesis of PGC91 strain using glucose as the substrate at 220 rpm. c Combinatorial overexpression of genes associated with the cytoplasmic reductive TCA cycle. Comparison of the physiological properties of the PGC91 (d) and PGC91-rT (e) strains during shaking-flask culture at 120 rpm. The initial concentration of glucose was 60 g/L. Data are presented as mean ± s.e.m. (n = 2 of b and c, 3 of d and e biologically independent samples). Statistical analysis was carried out by using Student’s t-test (one-tailed; two-sample unequal variance; *P < 0.05, **P < 0.01, ***P < 0.001). Source data are provided as a Source Data file.

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