Fig. 3: 6-phospho-gluconolactonase mutation redistributes the metabolic flux in PGC91-rTE strains. | Nature Communications

Fig. 3: 6-phospho-gluconolactonase mutation redistributes the metabolic flux in PGC91-rTE strains.

From: Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica

Fig. 3

a Protein structural analysis of Pgl1 mutants. b In vitro enzyme activity assay of Pgl1, Pgl1G75S, and Pgl1G74V. c In vivo NADH/NAD+ and NADPH/NADP+ ratios of PGC91-rT and PGC91-rTE strains. d Schematic position of the Pgl1 mutation in PPP and SA biosynthesis pathway. Zwf encoding glucose 6-phosphate dehydrogenase, Pgl encoding 6-phospho-gluconolactonase, Pgd encoding gluconate 6-phospho dehydrogenase. F6P fructose 6-phosphate, X5P xylulose 5-phosphate, S7P sedoheptulose 7-phosphate, GL6P gluconolactone 6-phospho, 6PG gluconate 6-phospho, Ru5P ribulose 5-phosphate, R5P ribose 5-phosphate, G3P glyceraldehyde 3-phosphate, and X5P xylulose 5-phosphate. NADH nicotinamide adenine dinucleotide, NADPH nicotinamide adenine dinucleotide phosphate. Data are presented as mean ± s.e.m. (n = 3 biologically independent samples). Statistical analysis was carried out by using Student’s t-test (one-tailed; two-sample unequal variance; *P < 0.05, **P < 0.01, ***P < 0.001). Source data are provided as a Source Data file.

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