Fig. 5: Optimization of reductive SA biosynthetic pathway in mitochondrial matrix of Y. lipolytica. | Nature Communications

Fig. 5: Optimization of reductive SA biosynthetic pathway in mitochondrial matrix of Y. lipolytica.

From: Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica

Fig. 5

a Metabolic reprogramming of mitochondria as an intracellular workshop for SA biosynthesis. Red arrows represent the oxidative TCA cycle, and blue arrows represent the reductive TCA cycle. mYlPyc encoding mitochondrial pyruvate carboxylase; YlMdh2 encoding malate dehydrogenase; YlFum encoding fumarase; mTbFrd encoding mitochondrial fumarate reductase; YlScs encoding succinyl-CoA synthetase; YlKgdh encoding α-ketoglutarate dehydrogenase. EMP Embden–Meyerhof–Parnas pathway. b SA titers and yields of mitochondrial localization of the enzymes in the reductive and oxidative TCA cycle in PGC91-rTE-mTbFrd strain. The initial concentration of glucose was 80 g/L. Data are presented as mean ± s.e.m. (n = 2 biologically independent samples). Statistical analysis was carried out by using Student’s t-test (one-tailed; two-sample unequal variance; *P < 0.05, **P < 0.01, ***P < 0.001; ns represents no significant difference). Source data are provided as a Source Data file.

Back to article page