Fig. 4: Chronic chemogenetic activation of the BLA-DMS circuit produces various compulsive-like phenotypes. | Nature Communications

Fig. 4: Chronic chemogenetic activation of the BLA-DMS circuit produces various compulsive-like phenotypes.

From: Persistent enhancement of basolateral amygdala-dorsomedial striatum synapses causes compulsive-like behaviors in mice

Fig. 4

a Experimental scheme for chronic BLA-DMS activation and diagrams of behavioral tests. Daily CNO (1 mg/kg) injections (i.p.) were administered for 12 consecutive days after 3 days of habituation period (Saline, n = 6; CNO, n = 6). b Number of repetitive checking (visits) to water or sucrose wells (Two-way repeated measures ANOVA, interactionday × drug, F13, 130 = 6.628, P = 1.360e−09). c Sucrose or water consumptions at baseline (BL) and Day 12 (D12). d Number of nose pokes (Two-way repeated measures ANOVA, interactionday × drug, F13, 130 = 6.740, P = 9.249e−10). e Number of rearing (two-sided paired t-test, Saline, t = 1.732, df = 5, P = 0.1438; CNO, t = 8.244, df = 5, P = 0.0004). f Grooming duration (two-way repeated measures ANOVA, interactionday × drug, F13, 130 = 2.272, P = 0.0099). g Number of checking occurrences on a reversal test (Two-way repeated measures ANOVA with post hoc Sidak’s multiple comparisons test, interactiondrink × drug, F1,10 = 10.10, P = 0.0099). h Left, representative images showing the collected food pellets (arrows) and beads (arrow heads). Right, quantification (Food pellets, two-sided Mann–Whitney test, U = 1.5, P = 0.0065; beads, unpaired t-test, t = 7.746, df = 10, P = 1.5603e−05). i Open arm duration in EPM (Saline, n = 5; CNO, n = 6) (two-sided unpaired t-test, t = 2.921, df = 9, P = 0.017).

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