Fig. 1: Mcam is a negative regulator of MECs and mammary gland development.
From: Mcam inhibits macrophage-mediated development of mammary gland through non-canonical Wnt signaling

a Schematic of screening strategy. Created with BioRender.com. b tSNE plot showing expression pattern of Mcam in different lineages according to single-cell sequencing data29. c qRT-PCR analysis of Mcam mRNA expression in different subpopulations of MECs shown in Figs. Sa-3b. n = 3 biological replicates. d Immunofluorescence (IF) images of Mcam co-stained with basal marker K14 and luminal marker K18. Nuclei were stained in blue by DAPI. Scale bar, 20 μm. e IF images of Mcam staining at various developmental stages of mammary gland. 4 W, 4-week old; I, involution. Scale bar, 20 μm. Representative images were taken from 3 mice for each stage. f qRT-PCR analysis of Mcam mRNA expression in MECs from WT and cKO mice by MMTV-Cre. n = 9 mice per genotype. g IF staining showing knockout efficiency of Mcam in WT and cKO mice. Scale bar, 20 μm. h Western blotting showing Mcam protein expression in WT and cKO mammary tissues. 3 mice for WT group and 4 mice for cKO group. i, j Representative images (i) and their quantification (j) of mammary duct extension of whole-mount staining in WT and cKO mice at 5 weeks of age. Arrow, length of epithelial extension. Scale bar, 5 mm. n = 10 mice in each group. k Representative images of mammary tissues in 5-week-old WT and cKO mice by H&E staining. Scale bar, 20 μm. l Quantification of mammary duct and TEB thickness in WT and cKO mice. n = 50 ducts or n = 30 TEBs in WT and cKO mice. Data are means ± SEM. Two-sided Student’s t test was used to evaluate statistical significance. Source data are provided as a Source Data file.