Fig. 7: Loss of Mcam promotes MEC proliferation via Ryk.

a tSNE plot showing expression pattern of Ryk in different lineages based on single-cell sequencing data²⁹. b tSNE plot showing expression pattern of Mcam (red), Ryk (green), and overlap (orange) in basal cells based on single-cell sequencing data⁴¹. c IF images of co-staining of indicated antibodies in WT mammary tissues. Scale bar, 5 μm. d qRT-PCR analysis of Ror2 and Ryk in MECs. n = 9 mice in each group. e Ryk expression levels in basal and luminal cells isolated from WT and cKO mammary tissues by MMTV-Cre. n = 3 biological replicates. f Western blotting showing Ryk protein expression in basal cells from WT and cKO mice. The experiment was repeated three times independently. g–i Representative images (g), clone numbers (h, n = 6 replicates for each group), and clone diameter (i, n = 10 replicates for each group) of colonies formed by WT and cKO mammry cells treated with anti-IgG or anti-Ryk, respectively. Scale bar, 100 μm. Data are means ± SEM. Two-sided Student’s t test was used to evaluate statistical significance. Source data are provided as a Source Data file.