Fig. 2: Endosperm cell types contain transcriptionally distinct subpopulations.
From: Decoding the gene regulatory network of endosperm differentiation in maize
a Visualization of five endosperm cell types by UMAP plots, colored according to cell clusters. b Heatmap depicting expression patterns of identified marker genes for major cell types. Selected classical marker genes and genes with known function are listed on the right. The colors from blue to red represent low to high expression levels. c Tree plot illustrating the relationship between clusters. d Representative GO terms enriched in different cell clusters. P-values were obtained using hypergeometric test, with g:SCS (graph-based stratified Cox-Snell) correction applied for multiple comparisons. e UMAP plots of gene-expression patterns related to auxin (AUX) and Jasmonic Acid (JA) biosynthesis and response. Colors ranging from gray to blue represent low to high expression levels. f, h Dot plots displaying selected markers of BETL and SE cells. f BETL, h SE. Circle size indicates the percentage of cells expressing the marker and color represents scaled expression values. g, i mRNA in situ hybridization results of genes shown in (f) and (h); scale bars, 100 μm. Experiments were repeated three times yielding similar results.
