Fig. 1: Optimization, construction, and characterization of a yeast strain with loxPsym sites distributed throughout the genome. | Nature Communications

Fig. 1: Optimization, construction, and characterization of a yeast strain with loxPsym sites distributed throughout the genome.

From: Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae

Fig. 1

a Distribution of 83 loxPsym sites in the SparLox83 strain. Detailed site locations are in Supplementary Data 1. The red diamond represents single loxPsym sites, while the red rectangle indicates multiple loxPsym sites located at the same loci as denoted within the dashed box. b SparLox83 phenotyping on various media. Ten-fold serial dilutions of overnight cultures of SparLox83R, SparLox83, parental strain JDY524, and wild-type BY4741 were analyzed on YPD, YPD + camptothecin (CPT, 8 μg/mL), and YPD + nocodazole (NDZ, 2.5 μg/mL). YPD, yeast extract peptone dextrose. Images taken after two days incubation at 30 °C. c PCR analysis of SparLox83 after ~125 generations to assess genomic stability. Potential loss of loxPsym was evaluated at 24 different loci in 35 passaged strains and no losses were observed. The frequency is a maximum estimate of loxPsym loss frequency per generation.

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