Fig. 6: Rapid improvement of acetic acid tolerance using genome-wide SCRaMbLE.
From: Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae
a Workflow of SCRaMbLE-Selection-based evolution cycles. Two heterozygous diploids, BY4741 × synIII (JDY546, red) and SparLox83R × synIII (JDY544, green), were inoculated into 48 individual wells and cycle growth-SCRaMbLE-screening was applied to SCRaMbLEant populations. Populations were screened after each round of SCRaMbLE by selection on YPD with an acetic acid (HAc) gradient. b After three rounds of evolution, SCRaMbLEant populations from SparLox83R × synIII were more resistant to HAc than those from BY4741 × synIII.
