Fig. 3: NAD+ recognition mechanism of SPARSA.
From: Structural basis of antiphage immunity generated by a prokaryotic Argonaute-associated SPARSA system
a The cryo-EM density of bound NAD+. b A cartoon representation of NAD+-bound in Sir2. NAD+ is shown as sticks colored in yellow by element, and the N-terminal Sir2 ___domain is colored in cyan and shown in surface mode. c An expanded view of the NAD+ interaction with SPARSA. The residues involved in NAD+ binding are shown in sticks. d The in vitro NADase assays of the wild-type and the variants of SPARSA. Each value is the mean ± SD and from more than three replicates.
