Fig. 4: The cryo-EM structure of SPARSA with gRNA/tDNA.
From: Structural basis of antiphage immunity generated by a prokaryotic Argonaute-associated SPARSA system
a The cryo-EM map (upper panel) and the structure of SPARSA with the gRNA/tDNA quaternary complex (bottom panel). b Schematic representation of the overall interactions between SPARSA and the gRNA/tDNA duplex. The bases involved in Watson–Cricks base pairing are indicated by red boxes and connecting lines. The gRNA and the tDNA are shown in the sticks and colored similar to a. The SPARSA are shown in cartoon mode and colored as in Fig. 1. c Recognition of the 5’ end of the gRNA of SPARSA. d The interaction between pAgo and the 3’-region of tDNA. e In vitro assays of the wild-type SPARSA system or the mutants on the 5’ end of the gRNA and the 3’-end of the tDNA. Each value is the mean ± SD and from more than three replicates.
