Fig. 6: GSDME disruption in neutrophils attenuated inflammation and alleviated lung injury during LPS-induced pneumonia.
From: Gasdermin E dictates inflammatory responses by controlling the mode of neutrophil death
a Schematic showing the experimental design for LPS-induced acute lung injury. b Quantification of total leukocytes and neutrophils in the BALF. All data are represented as mean ± SD, n = 4–5 mice/group, *P < 0.05 (WBC, P = 0.0247; Neutrophil, P = 0.0194). c Total protein in the BALF. Data are represented as mean ± SD, n = 4 mice/group, *P < 0.05 (P = 0.0350). d LDH release in the BALF. Data are represented as mean ± SD, n = 3–4/group, *P < 0.05 (P = 0.0201). e IL-6 and IL-1β levels in BALF. Data are represented as mean ± SD, n = 4 mice/group, *P < 0.05, **P < 0.01 (IL-6, P = 0.0309; IL-1β, P = 0.0033). f Hematoxylin and eosin (H&E) staining of lung sections. Results are representative of three biological replicates. g Lung inflammation and damage assessed as neutrophil recruitment (vol % of alveoli) and edema area of lung. Data are represented as mean ± SD, n = 4–5 mice/group, **P < 0.01 (Recruited neutrophils, P = 0.0072; Edema area of total area, P = 0.0058). h Experimental scheme for assessing LPS-induced pulmonary inflammation and associated lung injury in WT and neutrophil-specific Gsdme conditional KO mice. i Quantification of total leukocytes and neutrophils in the BALF. Data are represented as mean ± SD, n = 4–5 mice/group, *P < 0.05 (WBC, P = 0.0238; Neutrophil, P = 0.0276). j Total protein in the BALF. Data are represented as mean ± SD, n = 3–4 mice/group, *P < 0.05 (P = 0.0214). k LDH release in the BALF. Data are represented as mean ± SD, n = 3–4 mice/group, *P < 0.05 (P = 0.0487). l IL-6 and IL-1β levels in the BALF. Data are represented as mean ± SD, n = 3–4 mice/group, *P < 0.05, **P < 0.01 (IL-6, P = 0.0123; IL-1β, P = 0.0074). m H&E staining of lung sections. Results are representative of three biological replicates. n Pulmonary inflammation and tissue damage measured as neutrophil recruitment and edema area of lung. Data are represented as mean ± SD, n = 3 mice/group, **P < 0.01 (P = 0.0001). Statistical significance was examined by unpaired two-sided Student’s t test (b, c, d, e, g, i, j, k, l, n). Source data are provided as a Source Data file.
