Fig. 3: Single cell analysis of erythroid commitment within bone marrow and splenic HSPCs.

a UMAP embedding all of bone marrow and splenic HSPCs from control or PHZ-treated mice. Cells are labeled on the basis of their cluster identity (n = 4). The clusters correspond to hematopoietic stem cell (HSC), myeloerythroid progenitor (MPP-My/Ery), lymphoid-primed multipotent progenitor (MPP Ly), common lymphoid progenitor (CLP), erythroblast (EB), dendritic cell/macrophage progenitor (DC/Mac Prog), B cell/plasma cell progenitor (B/PC Prog), common myeloid progenitor (CMP), mast cell/basophil/eosinophil progenitor (MBE), NK/T cell progenitor (NK/T Prog), granulocyte/macrophage progenitor (GMP), T cell progenitor (T prog) as shown in (b). b Dot plot showing the average expression of selected marker genes in individual HSPCs. The size of dots represents the percentage of cells expressing a given gene. The cluster-wise mean expression value is shown in log scale and dots are colored on the basis of this value. c UMAP embedding HSPCs of each group. d Bar graph showing the distribution of transcriptional clusters of each group. e UMAP created by sub-clustering of the cluster MPP-My/Ery. Cells are divided into three clusters labeled as sub-cluster (SC) 1, 2, and 3. f The cells of clusters HSC, SC1-3, and EB are embedded based on their estimated psuedotime values. g Pseudotime trajectory of each group.