Fig. 5: Increased erythroid potential of splenic HSC during anemia depends on TET2. | Nature Communications

Fig. 5: Increased erythroid potential of splenic HSC during anemia depends on TET2.

From: Increased iron uptake by splenic hematopoietic stem cells promotes TET2-dependent erythroid regeneration

Fig. 5

a, b The numbers of HSCs (a) and relative changes of calcein AM MFI in HSCs (b) from bone marrow (left) or spleen (right) of wild-type or Tet2 knockout mice treated or untreated with PHZ (n = 2 for PHZ-treated wild-type in (b) and n = 3 for other groups in (a, b). c Transplantation of 50 splenic HSCs isolated from wild-type or Tet2 knockout mice treated or untreated with PHZ. Reconstitution level of erythroid progenitors were analyzed at 2 weeks post transplantation (n = 5 for wild-type and n = 4 for Tet2 knockout). The quantification of erythroid progenitor reconstitution levels is shown. d Colony forming assays with single-cell sorted bone marrow or splenic HSCs of wild-type or Tet2 knockout mice treated or untreated with PHZ. Frequency of colonies containing erythroid cells was shown (n = 2 for PHZ-treated wild-type bone marrow and n = 3 for other groups). e The frequency of erythroid progenitor cells (lin-Sca-1-c-kit+CD41-CD16/32-CD150-CD105+ cells [CFU-E+proE]) formed in vitro after 14 days culture of wild-type or Tet2 knockout bone marrow or splenic HSCs with or without PHZ treatment (n = 5, 5, 3, 3, 5, and 4 for untreated wild-type bone marrow, PHZ-treated wild-type bone marrow, PHZ-treated Tet2 knockout bone marrow, untreated wild-type spleen, PHZ-treated wild-type spleen, and PHZ-treated Tet2 knockout spleen, respectively). f The numbers of BMP4- and sonic hedgehog-responsive stress BFU-E colonies from wild-type or Tet2 knockout cells (n = 3). g Red blood cell count of control or PHZ-treated wild-type or Tet2 knockout mice 5 days after PHZ treatment (n = 3, 3, 5, and 4 for untreated wild-type, untreated Tet2 knockout, PHZ-treated wild-type, and PHZ-treated Tet2 knockout, respectively). The dotted line indicates the range of red blood cells considered normal. Comparisons were performed by unpaired, two-tailed Student’s t test. All data represent mean ± standard deviation. All numbers (n) are independent animals. Source data are provided as a Source Data file.

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