Fig. 1: Design of the BSV sensor for rapid detection of antibiotic resistance.
From: Rapid and visual identification of β-lactamase subtypes for precision antibiotic therapy
A The management of antibiotic resistance by conventional strategies and the BSV sensor. In most countries, healthcare institutions are commonly categorized into primary (no AST), secondary, and tertiary levels. Typically, suspected infection cases are initially managed to control symptoms in clinical settings based on the physician’s experience, and the medication would be adjusted according to the bacterial culturing results several days later. By contrast, the BSV sensor can report the β-lactamase subtypes of various body fluid samples from suspected infection patients within 0.25–3 h. B Design of the BSV sensor and working principle of the six independent sample chambers. Chamber 1 is loaded with tartrazine to reflect the quality of the BSV sensor, while chamber 2 makes use of bromophenol blue to indicate the presence of proteins. Chambers 3 to 6 are loaded with the four as-synthesized β-lactamase chromogenic probes to respectively differentiate BSBL, ESBL, AmpC, and carbapenemases. C Hydrolysis properties of the four β-lactamase subtypes towards the chromogenic probes.
