Fig. 2: Stepwise engineering of TnpB-associated ωRNA improved gene editing efficiency. | Nature Communications

Fig. 2: Stepwise engineering of TnpB-associated ωRNA improved gene editing efficiency.

From: Engineering a transposon-associated TnpB-ωRNA system for efficient gene editing and phenotypic correction of a tyrosinaemia mouse model

Fig. 2

a Reporter assay schematics of detecting cleavage activity of TnpB-ωRNA. b Predicted secondary structure of cognate ωRNA (231 nt). Cognate ωRNA was divided into 6 segments, named from S1 to S6. c Reporter assay results using engineered ωRNA by one-by-one truncation of S1 to S6. d Reporter assay results with engineered ωRNA by different combined truncations of S1 to S5. e Predicted secondary structure of a ωRNA-v1 variant with simultaneous truncation of S1, S2, and S3. f Reporter assay results for ωRNA variants with different SL deletion and modifications. g Predicted secondary structure of final optimized ωRNA-v2 (ωRNA*) variant. Data are represented as means ± SEM. A dot represents a biological replicate (n = 3). Source data are provided as a Source Data file.

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