Fig. 3: Characterization of endogenous gene editing activity and off-target effect with optimized TnpB-ωRNA system. | Nature Communications

Fig. 3: Characterization of endogenous gene editing activity and off-target effect with optimized TnpB-ωRNA system.

From: Engineering a transposon-associated TnpB-ωRNA system for efficient gene editing and phenotypic correction of a tyrosinaemia mouse model

Fig. 3

a The experimental workflow for detecting editing efficiency of original and optimized TnpB-ωRNA in HEK293T cells. b Gene efficiency comparison results for 14 human endogenous gene loci targeted by wild-type and optimized TnpB-ωRNA in HEK293T. c Gene editing activity comparison among TnpB-ωRNA, TnpB-ωRNA*, SaCas9 and SpCas9. All data points with median at center line and 25th and 75th quartile lines. Detailed statistical results in Source Data file. d Off-target analysis for top predicted off-target genomic loci via Cas-OFFinder. e Genome-wide off-target analysis with PEM-seq for the engineered TnpB-ωRNA. Data are represented as means ± SEM. A dot represents a biological replicate (n = 3 or more). Unpaired two-tailed Student’s t tests. Significant differences between conditions are indicated by asterisk (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, NS non-significant.). Source data are provided as a Source Data file.

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