Fig. 1: Activation of SHH signaling inactivates p38 to induce Gli1 protein. | Nature Communications

Fig. 1: Activation of SHH signaling inactivates p38 to induce Gli1 protein.

From: Phosphorylation of human glioma-associated oncogene 1 on Ser937 regulates Sonic Hedgehog signaling in medulloblastoma

Fig. 1

a, b Cerebella from GFAP-Cre;Smo-M2 pups bearing spontaneous MBSHH and from Smo-M2 control littermates at 2 weeks of age were harvested for quantitative iTRAQ LC-MS/MS phosphoproteomic analysis (n = 3 mice per group). Numbers of the differentially expressed phosphoprotein (a) and top candidates among them were listed (b). c H&E and immunohistochemistry staining of cerebella from above mice. Bar 200 μm. d Western blot analyses of cerebella from above mice (n = 3 mice per group). e Immunohistochemistry analyses for p-p38 and GLI1 in paraffin-embedded sections of non-WNT/non-SHH MB and MBSHH. Bar, 20 μm. f–h Western blot analyses in C3H10T1/2 cells treated with N-SHH (100 ng/ml), SB203580 (10 μM), PD98059 (10 μM), or SP600125 (10 μM) for the indicated times or 6 h. i–k Western blot analyses in C3H10T1/2 cells after transfection with the MAPK11, MAPK12, MAPK13 or MAPK14 construct for 48 h or after treatment with losmapimod at 10 μM for the indicated times. Data were presented as mean ± sd, two-tailed Student’s t test for (b). A representative example of three replicates is shown for c–k. Source data are provided as a Source Data file.

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