Fig. 3: Gli1(S941A) knock-in causes BCC-like proliferation in epidermis. | Nature Communications

Fig. 3: Gli1(S941A) knock-in causes BCC-like proliferation in epidermis.

From: Phosphorylation of human glioma-associated oncogene 1 on Ser937 regulates Sonic Hedgehog signaling in medulloblastoma

Fig. 3

a Western blot analyses of cytosolic versus nuclear Gli1 in cerebella of wild-type (WT), Gli1(S941A)+/-, Gli1(S941A)+/+, Gli1(S941E)+/- or Gli1(S941E)+/+ pups at 2 weeks of age. b, c Quantitative RT-PCR analysis for Ptch1 mRNA and co-immunoprecipitation experiments in cerebella of WT, Gli1(S941A)+/+ or Gli1(S941E)+/+ pups at 2 weeks of age (n = 3 mice per group). d Gross appearance of the cerebella and H&E staining for cerebellar sections from Gli1(S941A)-/- and Gli1(S941A)+/+ pups at 2 weeks of age. Bar, 200 μm. e Quantification of cerebellar weights and lobule number in Gli1(S941A)-/- and Gli1(S941A)+/+ pups (n = 6 mice per group). f Anti-Pax6, anti-NeuN and antin-Calbindin labeling in sagittal sections of the cerebellar vermis of Gli1(S941A)-/- and Gli1(S941A)+/+ pups. Bar, 20 μm. g Gross appearance of skin of Gli1(S941A)-/- and Gli1(S941A)+/+ mice at 4 months of age. h–k H&E staining and immunohistochemistry analyses and quantification for Gli1, Ki67, K5, K10, and K17 in skin sections of Gli1(S941A)-/- and Gli1(S941A)+/+ pups at 2 weeks of age (n = 3 mice per group), Bar, 50 μm. Data were presented as mean ± sd, two-tailed Student’s t test for (b, e, i and k). A representative example of three replicates is shown for (a, c, f and g). Source data are provided as a Source Data file.

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