Fig. 1: General Reference Matching Method. | Nature Communications

Fig. 1: General Reference Matching Method.

From: Multi-molecular hyperspectral PRM-SRS microscopy

Fig. 1

a A lipid subtype standard is analyzed by spontaneous Raman spectroscopy and preprocessed to generate a reference spectrum. b A sample is imaged using SRS to generate a HSI. c Each pixel of the HSI is a vector of intensity values that represent the Raman spectrum at that pixel. These spectra are compared using spectral angle mapping and illustrate how dissimilar spectra have a lower cosine similarity. d An example of a mouse brain sample with thresholded similarity scores with respect to sphingosine, cholesterol, and TAG. Pixel intensities are scaled to their similarity scores. SRS Stimulated Raman Scattering, HSI Hyperspectral Image, TAG triacylglyceride. (Panels (a) and (b) were created with BioRender.com).

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