Fig. 1: Increased linker EG repeats increases si < (EGXL)2 albumin binding by reducing self-association. | Nature Communications

Fig. 1: Increased linker EG repeats increases si < (EGXL)2 albumin binding by reducing self-association.

From: Structural optimization of siRNA conjugates for albumin binding achieves effective MCL1-directed cancer therapy

Fig. 1

Schematic structural representations of the siRNA and lipid components (a) and linker EG repeats (b) comprising the novel siRNA-lipid conjugates si < (EGXL)2. c Representative image of EMSA analysis, used to assess si < (EGXL)2 interaction with human albumin. d Representative in vitro binding responses of siRNA conjugates to human serum albumin (400 nM) measured by biolayer interferometry. e Model illustration of how micellization of siRNA-lipid conjugates, dictated by critical micelle concentration (CMC), influences availability of the lipids for albumin binding. f Critical micelle concentration of siRNA conjugates observed after 2 h of incubation with Nile Red at 37 °C. Each bar represents the average CMC ( ± S.D.), and each point represents the value measured for an individual experimental replicate, n = 3. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test.

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