Fig. 7: TCF-1 binds to Junb promoter and suppresses JunB expression in ILC3s. | Nature Communications

Fig. 7: TCF-1 binds to Junb promoter and suppresses JunB expression in ILC3s.

From: Dysregulation of Wnt/β-catenin signaling contributes to intestinal inflammation through regulation of group 3 innate lymphoid cells

Fig. 7

a, b ILC3s from Ctnnb1ex3fl/wt (Ctrl) and CreERT2Ctnnb1ex3fl/wt (KO) mice were treated with 4-hydroxytamoxifen. The chromatin accessible regions, histone modification marks, and TCF-1 binding peaks at Nfatc2 (a) and Junb (b) gene locus were detected by ATAC-seq, CUT&Tag assay and sequencing, as shown in the IGV browser view. c, d Luciferase activity in 293T cells transfected with vector alone (pGL3-vec), vector containing Nfatc2 locus N1 (pGL3-N1) (c) or vector containing Junb locus J1 (pGL3-J1) (d), together with empty vector (pcDNA-vec) or vector expressing TCF-1 (pcDNA-TCF-1). The results represent the relative firefly luciferase activities normalized to the corresponding Renilla luciferase activities. Each dot represents one individual replicate (n = 3 in c, d). Error bars represent the SEM. Statistical significance was tested by two-sided one-way ANOVA with Tukey’s test adjusted for multiple comparisons (c, d). Data are representative of three independent experiments (c, d).

Back to article page