Fig. 4: Phage resistance develops in vivo due to reduced phage adsorption.

a Heat map displaying phage resistance via efficiency of plating (EOP) of non-phage treated in vivo adapted isolates to the phages present in the cocktail. Each score represents susceptibility (white squares, score = 1) to resistance (dark blue squares, score = 0), n = 3 for each group. b Heat map illustrating phage resistance (via EOP) of isolates recovered from delayed PELP20 treated mice to the phages in the cocktail. Scores range from complete susceptibility (white squares, score = 1) to complete resistance (dark blue squares, score = 0), n = 3 for each group. c Heat map showing phage resistance (via efficiency of plating) of isolates recovered from delayed phage cocktail treated mice to the phages present in the cocktail. Scores range from complete susceptibility (white squares, score = 1) to complete resistance (dark blue squares, score = 0), n = 3 for each group. d Phage cocktail adsorption over time for the input isolate (black circles) and non-phage treated in vivo adapted isolates from the lungs (pink circles), liver (green circles), and kidney (purple circles). Mean with SEM is indicated, n = 3 for each group. e Phage cocktail adsorption over time for the input isolate (black circles) and phage cocktail treated in vivo adapted isolates from the lungs (pink circles) and liver (green circles). Mean with SEM is indicated, n = 3 for each group. Source data are provided as a Source Data file.