Fig. 1: Complexity and magnitude of circulating antibody-secreting cells in active SLE. | Nature Communications

Fig. 1: Complexity and magnitude of circulating antibody-secreting cells in active SLE.

From: Distinct transcriptomes and autocrine cytokines underpin maturation and survival of antibody-secreting cells in systemic lupus erythematosus

Fig. 1

Peripheral blood mononuclear cells (PBMCs) were isolated from steady-state healthy donors, influenza vaccinated heathy subjects on day 7 post immunization, active SLE patients, or inactive SLE patients. Isolated cells were stained and analyzed by flow cytometry. a Representatives plots of the frequencies of CD19+ ASC (CD27++ CD38++) in the CD3- CD14- CD19+ gate. b Gating strategy for the identification of ASC populations Pops 2-5, based on the CD19+ and CD19- gate, in active SLE patients (left) and heathy subjects on day 7 post influenza immunization (right). c Expression of surface CD138 on ASC populations from active SLE patients (n = 21) and influenza vaccinated heathy subjects (n = 20). Histograms (top), illustrate representative examples and box plots include compiled data (bottom). Data are shown as mean ± SEM. Statistical significance between SLE patients and vaccinated healthy subjects was assessed using Student’s t test. d Frequencies of total ASC, CD19+ ASC, and CD19− ASC in total B cells and ASC combined (top) from steady-state healthy donors (n = 19), influenza vaccinated heathy subjects (n = 33), active SLE patients (n = 63), or inactive SLE patients (n = 41). Numbers of total ASC, CD19+ ASC, and CD19− ASC per μL of the peripheral blood (bottom) from steady-state healthy donors (n = 14), influenza vaccinated heathy subjects (n = 17), active SLE patients (n = 30), or inactive SLE patients (n = 27). Data are shown as mean ± SEM. e, Frequencies of each ASC Pops 2–5, in the total B cells and ASC combined (top) from steady-state healthy donors (n = 19), influenza vaccinated heathy subjects (n = 33), active SLE patients (n = 63), or inactive SLE patients (n = 41). Numbers per μL of the peripheral blood (bottom) from steady-state healthy donors (n = 14), influenza vaccinated heathy subjects (n = 17), active SLE patients (n = 30), or inactive SLE patients (n = 27). Data are shown as mean ± SEM. Statistical significance was assessed using Kruskal–Wallis test followed by Dunn’s test for multiple pairwise comparisons (d, e). p values are shown on plots. Source data are provided as a Source Data file.

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