Fig. 6: OC2 can globally stimulate HSV-1 gene transcription. | Nature Communications

Fig. 6: OC2 can globally stimulate HSV-1 gene transcription.

From: Neuronal miR-9 promotes HSV-1 epigenetic silencing and latency by repressing Oct-1 and Onecut family genes

Fig. 6

a Neuro-2a cells were transfected with 200 ng/ml plasmid for 42 h, then infected with ICP0-null virus (MOI = 1) in media containing 100 μM ACV or DMSO for 3 or 6 h before RT-qPCR analysis. b Neuro-2a cells were transfected with 200 ng/ml plasmid, then infected with ICP0-null virus (MOI = 1) for 3 h before viral DNA quantification. c Neuro-2a cells were transfected with 200 ng/ml plasmid for 42 h, then infected with ICP0-null virus (MOI = 2) for 4 h before RNA-seq analysis. Normalized reads for each viral transcripts were log2 transformed before illustration by the Heatmap. The right panel shows the fold changes for genes of different kinetic classes with each point representing a viral transcript. n = 3 (a, c) or 4 (b) biologically independent samples. Data were analyzed by two-way ANOVA with Sidak’s multiple comparisons tests (a) or two-sided unpaired t tests (b) and are presented as the mean ± s.d. Source data are provided as a Source Data file.

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