Fig. 6: Characterization of SenB-like enzymes. | Nature Communications

Fig. 6: Characterization of SenB-like enzymes.

From: Substrate binding and catalytic mechanism of the Se-glycosyltransferase SenB in the biosynthesis of selenoneine

Fig. 6

a Protein sequence comparison of CbSenB, RsSenB and SenB. N20/T23, K158 and the EGGAHV motif are labeled with black triangles. b–e HPLC-UV/DAD analysis of the catalytic activities of CbSenB and RsSenB using four different UDP-sugars. f Overall structure of RsSenB. Two molecules within one asymmetric unit are shown. g Spatial positions of K158 in SenB (cyan) and RsSenB (magenta). h HPLC-UV/DAD analysis of the catalytic activities of putative catalytic residue mutants of RsSenB and CbSenB using UDP-Glc as the sugar donor.

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