Fig. 3: GSNO targets STING. | Nature Communications

Fig. 3: GSNO targets STING.

From: S-nitrosothiol homeostasis maintained by ADH5 facilitates STING-dependent host defense against pathogens

Fig. 3

ELISA analysis of cGAMP production in HSV-1-infected (a) and ISD-stimulated (b) PMs pretreated with solvent (Ctrl) or GSNO. c Co-immunoprecipitation analysis of cGAMP-biotin binding to STING in HSV-1-infected PMs pretreated with solvent (Ctrl) or GSNO. d Immunoblot assays of dimer-STING in cGAMP-stimulated PMs pretreated with solvent (Ctrl) or GSNO. Immunoblot assays of Oligo-STING in HSV-1-infected (e) and cGAMP-stimulated (f) PMs pretreated with solvent (Ctrl) or GSNO. g Confocal microscopy analysis of the co-localization of STING (green) and cis-Golgi (GM130, red) in BJ cells pretreated with solvent (Ctrl) or GSNO and then stimulated with cGAMP. The intensity profiles of each line were quantified by Image J software. h Colocalization analysis of STING and cis-Golgi (GM130) by Manders’ Colocalization Coefficients (MCC). i ELISA analysis of interferon (IFN)-β secretion in PMs from Sting+/+ or Sting-/- mice pretreated with solvent (Ctrl) or GSNO, plus stimulation as indicated. j qPCR analysis of Ifnb mRNA expression in PMs from Sting+/+ or Sting-/- mice pretreated with solvent (Ctrl) or GSNO and then stimulated with HSV-1, LPS or poly(I:C). k qPCR analysis of IFNB mRNA expression in THP1 cells pretreated with solvent (Ctrl) or GSNO and then stimulated with HSV-1, cGAMP, poly(I:C) or VSV. Data represent mean ± SD or one representative image from three independent experiments. The p values were calculated using unpaired two-sided t test and adjustments were made for multiple comparisons.

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