Fig. 5: Homo-BacPROTACs degrade intracellular ClpC1. | Nature Communications

Fig. 5: Homo-BacPROTACs degrade intracellular ClpC1.

From: Homo-BacPROTAC-induced degradation of ClpC1 as a strategy against drug-resistant mycobacteria

Fig. 5

a Intracellular degradation of ClpC1 in Msm #607 cells following a 24 h incubation with Homo-BacPROTACs 8 (UdSBI-0545), or 12 (UdSBI-4377). Green vertical lines indicate the DC50 values obtained in that particular experiment. b ClpC1 levels in Msm #607 following a 24 h incubation with distomers 8a (UdSBI-0966), or 12a (UdSBI-0117). c ClpC1 levels in Msm #607 following a 24 h incubation with monomers 5 (green), 10 (blue), or dCymC (red). The curves show representative experiments performed in well triplicates. Experiments for each compound were performed independently n = 3 (compounds 8, 8a, 12a, dCymC, 5, 10) or n = 4 (compound 12) times with similar results. Error bars indicate mean ± SD of n = 3 well replicates for that given experiment. The actual mean DC50 values reported in the text for compound 8 or 12 are calculated from the respective individual DC50 values obtained for the independent cellular degradation experiments conducted in this study and are summarized in the Source Data file for Fig. 5a. Source data are provided as a Source Data file.

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