Fig. 2: Both Pcdha9 Mut and Del mice exhibit progressive motor function deficits.
From: PCDHA9 as a candidate gene for amyotrophic lateral sclerosis
a Hemiplegia in the left hind limb of 14-month-old Mut mice. b Survival curves (WT, n = 84; Mut, n = 99; Del, n = 109. n represent biologically independent replicates). A Few Mut and Del mice started to die at 5 months old, and the death rate progressively worsened. c, d Abnormal footprints detected in both Mut and Del mice (12-month-old). Stride length (cm) and stance of limbs (left, right, hind limb and forelimb) tested by footprint tests as shown in (c) and analyzed at different ages (month) (HS: Hind limb stance, FS: Forelimb stance, LH: Left hind limb, LF: Left forelimb, RH: Right hind limb, RF: Right forelimb). The mouse cartoons showed the measurement diagram (red: forepaws; blue: hind paws). e Grip force (gram) for limbs of the mice at 8-, 10- and 12-month old. f Rota rod test performed in five consecutive days. Time until falling was analyzed. n = 8 in WT, n = 5 in Mut group and n = 6 in Del group, n represents biologically independent replicates. P values with blue color represent p between WT and Mut; P values with brown color represent p between WT and Del. g Time crossing the swimming tank in the swimming test. h Statistical graph of the hind limb abnormal movement of claws in 10-month and 12-month old mice. All the mice used for behavior test are male mice. All data represent mean ± SEM. one-way ANOVA. For the behavior tests above, each group n ≥ 4 biologically independent replicates, the specific numbers are provided in source data. Source data are provided as a source data file.
