Fig. 7: Na⁺/K⁺ ATPase-α1 expression in spinal motor neurons is significantly downregulated in Pcdha9 mutant mice.

a Co-localization (white arrow) of overexpressed hPCDHA9 with endogenous Na⁺/K⁺ ATPase-α1 on the plasma membrane in 293 cells. Scale bar: 5 μm. b Endogenous Na⁺/K⁺ ATPase-α1 interacts with overexpressed WT and mutant hPCDHA9 in 293 cells. Anti-FLAG immunoprecipitation was performed. The input and immunoprecipitated proteins were detected by Western blots. Uncropped blots are shown in Source Data. 3 independent repeats showed similar results in (a) and (b). c Confocal images of Na⁺/K⁺ ATPase-α1 expression pattern in the lumbar spinal cord of 12-month-old mice (crossed with HB9-GFP mice). The signal of Na⁺/K⁺ ATPase-α1 is strong in the motor neurons. scale bar: 100 μm. Na⁺/K⁺ ATPase-α1 (red). d Expression of Na⁺/K⁺ ATPase-α1 in 12-month-old mice lumbar spinal cord. t-test. The statistic data represent mean ± SEM. n = 4 biologically independent replicates. All uncropped blots were provided in Source Data. e Enlarged images of Na⁺/K⁺ ATPase-α1 in ventral horn of spinal cord (L3–L5), the signal in motor neurons (marked by CHAT) is decreased in Mut mice. n = 3 each in WT and Mut mice, n represent the biological repeats. Scale bar for low power images were 20 μm and for enlarged images were 10 μm. Source data are provided as a source data file.