Fig. 3: The dynamic changes of CDK1 activation and recovery after hyperthermia.

a Representative western blot image with indicated proteins, delineating the dynamic changes in CDK1 activity at different recovery times post-hyperthermia (HT). OVCAR8, A2780, and ID8 cells were treated with HT (42 °C) incubation for 90 min, then were allowed to recover at 37 °C for the indicated times (0, 0.5, 1.5, 3, 6, 12, 24, and 48 h). b, c γH2AX, EdU, and PI staining were analyzed by flow cytometry in OVCAR8 cells that were treated with HT for 90 min and allowed to recover at 37 °C for the indicated times (n = 3 per group). d Representative images and quantification of BrdU and γH2AX positive OVCAR8 and A2780 cells treated with HT for 90 min, followed by recovery at 37 °C for the indicated times, are presented (n = 3 per group). Scale bar, 25 μm. Comparisons were performed by unpaired two-tailed Student’s t test in (c) and one-way ANOVA followed by Dunnett’s multiple comparisons test in (d). Data are presented as mean ± SEM. ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05, ns., not significant. All analytical data are derived from a minimum of three biologically independent experiments, with “n” indicating the specific number of replicates. Source data are provided as a Source Data file.