Fig. 1: Cryo-EM structure of S. cerevisiae Doa10 in detergent micelle.
From: Substrate recognition mechanism of the endoplasmic reticulum-associated ubiquitin ligase Doa10
a Schematic diagram of the ___domain structure of S. cerevisiae Doa10. Helices are represented as cylinders (TMs 1-14 are numbered). Numbers in gray indicate amino acid residue number. b Cryo-EM density map of Doa10 viewed from the cytosol. Shown is a high-resolution protein density map overlaid with a lowpass-filtered detergent micelle density (gray). The color scheme is the same as in (a). c As in (b), but showing a side view along the membrane plane. d As in (c), but overlaid a density map with a lower surface threshold to show RING-CH and L2/3 features. e, f Atomic model of Doa10 based on the cryo-EM map. Views in (e) and (f) are equivalent to (b) and (c), respectively. Parts that are unresolved in cryo-EM were schematized.
