Fig. 7: Oprm1 effect in a glaucoma model.

a Experimental model for the elevation of intraocular pressure (IOP) induced by intracameral injection of magnetic microbeads. A representative photo shows magnetic microbeads stuck between the circumference of the cornea and iris after injection. b IOP values across 8 weeks. Data are presented as mean ± SEM. Sham group, n = 4 mice; eIOP group, n = 4 mice; Oprm1 + eIOP, n = 5 mice. One-way ANOVA. p-values: 0.4588 (wk 0), 0.0004 (wk 1), 0.0009 (wk2), <0.0001 (wk 3), <0.0001 (wk 4), <0.0001 (wk 5), <0.0001 (wk 6), <0.0001 (wk 7), 0.0002 (wk 8). c and d Protection of RGCs from eIOP with overexpression of Oprm1. Data are presented as mean values ± SEM. n = 4 or 5 mice for each independent experimental condition. e protection of axons in optic nerve with overexpression of Oprm1. f Quantification of axon survival, shown as percentages of axons relative to those in the sham group. Data are presented as mean values ± SEM. n = 4 or 5 mice for each independent experimental condition. g–j Assessment of Oprm1 overexpression on visual function using the vision cliff arena recording. g Representative movement heatmaps from sham, eIOP, and Oprm1+eIOP. h Quantification of time spent on the shallow side during the 30 minutes in the test. i A schematic diagram and representative traces (5 min) of the visual cliff test. Red traces highlight the pass-through from the shallow to the deep side, while blue traces highlight the retreat to the shallow side. j Percentages of retreat events among all traces entering the boundary zone during the 30 min. Data are presented as mean ± SEM. Sham group, n = 7 mice; eIOP group, n = 4 mice; Oprm1 + eIOP, n = 5 mice. One-way ANOVA, multiple comparisons. Scale bars: 50 μm (c), 10 μm (e). Source data are provided as a Source Data file.