Fig. 5: In vivo activatable afterglow imaging of orthotopic liver tumors. | Nature Communications

Fig. 5: In vivo activatable afterglow imaging of orthotopic liver tumors.

From: Acidity-activatable upconversion afterglow luminescence cocktail nanoparticles for ultrasensitive in vivo imaging

Fig. 5

a Schematic illustration of the detailed procedures of hepatocellular carcinoma in situ through the afterglow imaging. b Afterglow images under an 808 nm pre-irradiation (left panel), under white light pre-irradiation (middle panel) and fluorescence images (right panel) after intravenous injection of ALCNs or C-ALCNs (4 mg/kg ASN or C-ASN, and 0.4 mg/kg AIN). The fluorescence images were acquired with an excitation wavelength at 480 nm and emission wavelength at 580 nm. The quantified SNRs for afterglow luminescence imaging under 808 nm pre-irradiation (c), under white light pre-irradiation (d), fluorescence imaging (e) of orthotopic liver tumor-bearing mice as a function of time. f Ex vivo afterglow images of various tissues under an 808 nm and white light pre-irradiation from an orthotopic liver tumor-bearing mice at 48 h post- injection of ALCNs or C-ALCNs intravenously. g Quantification of ex vivo afterglow intensities of various tissues in (f). h H&E staining images of tumor slices of mice after injection with ALCNs or C-ALCNs (N: normal tissue, T: tumor tissue). The scale bar represents 100 μm. Data are presented as mean  ± s.d. and analyzed by the one-way ANOVA (n = 3 mice each group). Source data are provided as a Source Data file.

Back to article page