Fig. 5: Tumor-infiltrating lymphocyte analysis.

The radiographic tumor response was significantly associated with the densities of tumor-infiltrating CD8 + T cells (n = 47, Spearman R = 0.405, P = 0.0005) (a) and tumor-infiltrating M1-like macrophage cells (n = 47, Spearman R = 0.375, P = 0.009) (b) by using the two-sided Spearman correlation text. The line represents a fitted line. The gray shadow represents the corresponding 95% confidence interval. The baby-blue dot represents an individual data point. The multiplex immunofluorescence images of the tumor sites in patient 20 (c) and patient 45 (d). Primary antibodies targeting CD8, CD56, CD68, HLA-DR, and Pan-CK were used on the same slide. Comparisons of the densities of tumor-infiltrating CD8+ cells (P < 0.0001) and M1-like macrophage cells (P = 0.01) between HPV-positive (n = 9) and HPV-negative (n = 38) patients (e); CD8+ cells (P = 0.0002) and M1-like macrophage cells (P = 0.0028) between TP53-mutant (n = 37) and TP53-wild-type (n = 10) patients (f), and CD8+ cells (P = 0.19) and M1-like macrophage cells (P = 0.07) between TERT-mutant (n = 11) and TERT-wild-type (n = 36) patients (g) analyzed by using the two-sided unpaired t test. Box-and-whisker plots show the distribution (box, whiskers, and outliers). The center line represents the median, the box limit represents the interquartile range (IQR), the whiskers represent the 1.5×IQR, and the outliers represent an individual data point. *P < 0.05, **P < 0.01, ***P < 0.001, ns no significance. Source data are provided as a Source Data file.